A continuous region of coding sequence can be PCR amplified using a 5′ primer possessing a polymerase binding site and a translation initiation site in-frame with the 5′ end of the test sequence. The ...

Background: Bloodstream infection is a major cause of morbidity and mortality in hospitalized patients worldwide. Delays in the identification of microorganisms often leads to a poor prognosis. The ...

Rapid identification and susceptibility testing are still major challenges in recent medical microbiology, in particular in the era of multi-/pan-resistant microorganisms. DNA/RNA-based molecular ...

In diagnostics of infectious diseases, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) can be applied for the identification of pathogenic microorganisms.

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) represents the successful combination of retentate chromatography and mass spectrometry, and this ...

Using MALDI-TOF, researchers can quickly and accurately determine polymer, protein and nucleic acid masses. They can also analyze and resolve complex assortments of smaller peptides and ...

Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories for bacterial identification but its use for detection ...

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The inimitable adaptability of MALDI-TOF/TOF systems enables tissue imaging of intact proteins, as well as of tryptic peptides brought about by on-tissue digestion. Although intact protein imaging can ...

What is time-of-flight mass spectrometry? How ToF MS has gained more interest since the commercialization of MALDI. Why a high-speed ADC is key to ToF MS performance. Mixed-signal front end that ...

Antonation KS, Tracz DM, Dreger AD, Corbett CR. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based identification of security-sensitive bacteria: Considerations for ...